Sequencing the V4 region of 16S. Sequence variation in the 16S ribosomal RNA (rRNA) gene is widely used to characterize taxonomic diversity present in microbial communities. The 16S sequence is composed of nine hypervariable regions interspersed with conserved regions.
Paired-end sequencing was performed with a 250-bp length for the V1-V2 region (V12) and with a 300-bp length for the V3-V4 region.
Analysis of v3-v4 region of filtered reads indicates that species are placed in 9 phyla, 15 classes, 20 orders, 20 families, 20 genus's and 20 species. Among them most dominant phylum was firmicutes having 51% diversity and Chloroflexi (0.01%) had minimum domination.
The main reason for using V3/V4 region in majority of the cases that these regions contain the maximum nucleotide heterogeneity and displays the maximum discriminatory power.
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