Metagenomic NGS (mNGS) is simply running all nucleic acids in a sample, which may contain mixed populations of microorganisms, and assigning these to their reference genomes to understand which microbes are present and in what proportions.
In genetics, shotgun sequencing is a method used for sequencing random DNA strands. It is named by analogy with the rapidly expanding, quasi-random shot grouping of a shotgun.
Shotgun sequencing had a number of important advantages over previous methods: Faster because the mapping process was eliminated. Uses less DNA than other methods. Less expensive than approaches requiring a map.
Shotgun metagenomic sequencing allows researchers to comprehensively sample all genes in all organisms present in a given complex sample.
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